Abstract
Chlorophyll was bleached when leaves of various species were incubated in aq. acetone solutions at pH 6 in the dark, but not when they were suspended in solutions of several surface-active compounds. Leaves that bleached in aq. acetone were all rich in lipoxidase but a few with high activity did not bleach appreciably. Although leaves with low lipoxidase activity did not bleach, pheophytin or pheophorbide was sometimes formed and decreased the absorption of pigment-containing extracts. Adding glycollate to chloroplast-containing aqueous extracts of wheat and barley leaves caused chlorophyll to bleach, but glycollate oxidation seems not to be involved in the bleaching in leaves in aq. acetone.
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