Abstract
We previously demonstrated that quercetin may protect against colon cancer via inhibition of pro-inflammatory molecules. The goal of this study was to determine the capabilities of quercetin and chlorogenic acid, stone fruit bioactives, to reduce inflammation in a colitis model. Sprague Dawley rats (n=63) received diets (control, 0.05% chlorogenic acid, or 0.45% quercetin) for 3 wk before dextran sodium sulfate (DSS) (3%, 3x, 14 day separation)(n=11/diet) or control (no DSS, n=10/diet) treatments. Colons were assessed for injury and inflammation, while NF-κB activity was measured in scraped mucosa. Fecal moisture content (48.7%) was similar among diets before DSS treatment. DSS increased (p<0.0001) fecal moisture, and elevated injury (p<0.0001) and inflammation (p<0.05). Chlorogenic acid resulted in lower injury (p<0.05) and decreased NF-κB activity (p<0.05) in DSS treated rats. Fecal quercetin concentrations were unaffected by DSS (14.5 mg/g), while fecal chlorogenic acid was not detectable. Although DSS elevated injury, inflammation, and NF-κB activity in all groups, chlorogenic acid reduced DSS-induced injury and NF-κB activity. Funded by USDA/NIFA 2008-34402-19195 and 2009-34402-19195.
Published Version
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