Abstract
The aim of this study is to investigate the effect of chlorogenic acid (CA) on 3T3-L1 adipocytes and its mechanism action. CA at 1-25 μM showed no cytotoxicity to 3T3-L1 pre-adipocytes and 3T3-L1 adipocytes. CA significantly inhibited oil red O-stained material (OROSM) and intracellular triglyceride levels in 3T3-L1 adipocytes in a concentration-dependent manner. In addition, CA down-regulated the glycerol-3–phosphate dehydrogenase (GPDH) and peroxisome proliferator–activated receptor γ (PPARγ) activity. A real-time polymerase chain reaction revealed that CA inhibited the PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), sterol regulatory element binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) gene expression, which may in part account for anti-adipogenesis of CA. Thus, CA could act as a potential lipid lowering functional resource.
Highlights
A WHO survey indicates that in 2020, worldwide obesity has nearly triple since 1975
The aim of this study is to investigate the effect of chlorogenic acid (CA) on 3T3-L1 adipocytes and its mechanism action
The result indicated that CA at 1-25 μM exhibited no cytotoxicity to 3T3-L1 preadipocytes after 24 h of incubation with CA
Summary
A WHO survey indicates that in 2020, worldwide obesity has nearly triple since 1975. Most of the world’s population live in countries where overweight and obesity kills more people than underweight [1]. Djulis has been reported to be effective in the inhibition of adipogenesis in 3T3-L1 cell [3], and an in vivo study has shown that oral administration of djulis has beneficial effects on hyperlipidemia in obese mice [4]. Some reports have noted that bioactive compounds, such as flavonoids, may cause an inhibition of adipogenesis and lipogenesis, and induction of lipolysis as well as apoptotic death of adipose cells [5] These findings imply that complementary and alternative therapies with functional biomaterials can regulate lipid metabolism and prevent overweight and obesity. The investigations of natural products or bioactive compounds for preventing and treating overweightness and obesity have received a great deal of attention
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