Chlorella vulgaris (Chlorophyta) Suşlarının Kesikli Kültür Sisteminde Yığın Kültürlerinin Üretimi Üzerine Bir Çalışma

Abstract

In this study, it is aimed to isolate Chlorella vulgaris strain, a single-celled green algae that is common in freshwater deposits, to establish culture conditions and to obtain batch cultures. C. vulgaris has been used in food, cosmetics and pharmaceutical industries as well as become encouraging for the production of biodiesel and the treatment of wastewater in recent years. C. vulgaris strains isolated from freshwater deposits were cultivated in a semi-continuous culture system of 100, 200, 500, 1000, 2000 and 4000 ml. Subsequently growth factors such as initial inoculum amount of the strains, appropriate pH, temperature and ventilation were tested in order to optimize the conditions. The highest value in the cell counts of 4000 ml batch cultures at the 120 th hours was determined as 6.8x10 4 h/ml in AUFFH strain respectively the others were as follows: TOH 6.7x10 4 h/ml, UIK 6.3x10 4 h/ml, GURH 6.2x10 4 h/ml and GUMSH 6.1x10 4 h/ml. The highest amount in terms wet weight for 4000 ml batch cultures was obtained as AUFFH 152 (g/2000 ml), this value was followed by TOH 129.6 (g/2000 ml), GURH 120.8 (g/2000 ml), GUMSH 84.3 (g/2000 ml), UIK 48.8 (g/2000 ml).