Abstract
Nowadays, nanotechnology contributes diminishing side effects rather than traditional therapeutic methods like chemotherapy. Thus, designing a biocompatible specific targeted nanocarrier with prolonged half-life and enhanced bio-availability using simultaneous cell imaging seems urgent. To meet this demand, 5-fluorouracil-chitosan‑carbon quantum dot-aptamer (5-FU-CS-CQD-Apt) nanoparticle was successfully synthesized for specific targeted delivery of 5-FU anti-cancer drug used in breast cancer treatment and this was done by following facile water-in-oil (W/O) emulsification method. Physicochemical properties were characterized and high drug loading and entrapment efficiency were achieved. The average size and zeta potential of the nanoparticle were 122.7 nm and + 31.2 mV, respectively. According to the in-vitro drug release profile, 5-FU-CS-CQD-Apt released the drug in a controlled manner. MTT assay, flow cytometry, fluorescence microscopy, and gene expression results demonstrated that the blank nanoparticle was biocompatible, and 5-FU-CS-CQD-Apt could kill tumor cells efficiently. Bcl-2/Bax ratio was decreased after 5-FU-CS-CQD-Apt treatment in MCF-7 cells. It was concluded that 5-FU-CS-CQD-Apt could be used as a potential nanocarrier in breast cancer treatment.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Biological Macromolecules
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
