Chitosan nanoparticles augmented indole-3-acetic acid production by rhizospheric Pseudomonas monteilii.

Abstract

Rhizospheric Pseudomonas spp. are widely used for upgrading sustainable agriculture because of their ability to execute multifaceted plant beneficial functions. In the current study, chitosan nanoparticles (CNPs) were used to analyze their effect on plant beneficial properties of rhizospheric Pseudomonas monteilii. The CNPs were characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis. The impact of CNPs on indole-3-acetic acid (IAA) production of P. monteilii was analyzed and quantified by spectrophotometric and confirmed high-performance liquid chromatography analysis. This revealed the beneficial effect of CNPs (1 mg/ml) by enhancing the IAA production of P. monteilii. In planta effect of varied bacterial IAA production was further demonstrated in Vigna unguiculata. Here, enhancement in shoot length (35.79 ± 0.37 cm), leaf number (7 ± 0.54), and fresh weight (3.07 ± 0.11 g) were observed in the plants treated with the culture filtrate collected from P. monteilii cultivated with 1 mg/ml CNPs. The results of the study highlight the beneficial effect of the CNPs to augment the rhizobacterial functioning by inducing the expression of plant beneficial properties.