Abstract
The paper presents the evaluation of chitosan and carboxymethyl chitosan matrix on the gold film of surface plasmon resonance (SPR) chips for the selective detection of ochratoxin-A (OTA) via immobilization of ochratoxin-A-bovine serum albumin (OTA-BSA) conjugate. Surface modification with flexible chitosan-based nanomatrix provides fast and efficient binding of the conjugates. The binding between OTA-BSA conjugate and chitosan was analyzed by localized Fourier transformation infrared reflectance (FTIR) spectroscopy followed by surface plasmon resonance. A clear SPR shift together with the presence of amino (N-H) functional groups on the surface confirmed the binding of conjugates with chitosan and carboxymethyl chitosan. The covalent binding on the surface resulted in a stable and resistant surface to increase the sensitivity of the SPR-based sensor. The limit of detection for OTA on chitosan-based SPR sensor was found to be around 2.52 ng/mL.
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