Abstract
The bone repair process, among other physiological mechanisms, occurs in a harmonious manner throughout the body. However, the presence of some deleterious factors such as surgery, trauma or pathology, can interfere with the physiology of bone remodeling. It´s Known that the synergistic antimicrobial action between drugs and biomaterials can help and favor osteogenesis after grafting surgery. Therefore, this study aims to develop chitosan / hydroxyapatite scaffolds with amoxicillin for bone repair in the oral cavity. For this, raw materials were selected and characterized and scaffolds were made by the processes of solubilization, dispersion and lyophilization. The characterizations were made by optical microscopy, scanning electron microscopy, eenergy dispersive spectrometer, swelling potential, degradation analysis, apparent porosity test and in vitro cell cytotoxicity. The scaffolds produced in this research showed to have not only adequate physical characteristics but also chemical and biological ones. It was as well as detected a reproducible methodology that resulted in a biomaterial with morphology that provided a high degree of swelling, porosity, satisfactory degradation and the presence of amoxicillin which was confirmed by the eenergy dispersive spectrometer. Nevertheless, the scaffolds with 30% hydroxyapatite showed the best results for in vitro cell viability. So, it is possible to conclude that the scaffolds produced show successful characteristics for bone repair in the oral cavity.
Highlights
Thousands of bacteria live in the human body and are everywhere
The shape of the scaffolds was determined by the chitosan matrix that maintained its morpho structural characteristics even after the dispersion of hydroxyapatite and amoxicillin particles
Among the other samples the standard deviation was not significant. All these results show that the microstructural characteristics of the scaffolds produced in this research are similar and reproducible according to the results found in OM, SEM and porosity test
Summary
Thousands of bacteria live in the human body and are everywhere. In the oral environment, for example, around 500 species that are part of the oral microbiota coexist. Surgery, trauma, diseases, poor hygiene, inadequate diet, among other circumstances can cause imbalance in this habitat In such situations, a selection occurs for certain opportunistic pathological microorganisms and the biofilm becomes virulent and may cause local and systemic lesions that trigger deleterious effects throughout the organism (Nicolas & Lavoie, 2011; Sixou, Diouf, & Alvares, 2007). The control of virulence biofilm has such a great importance for the treatment, prevention and reestablishment of altered normal functions. In this sense, many biomaterials have been investigated in an attempt to aid and protect, for example, bone repair in injured or surgery regions (Khanna et al, 2017; Shu et al, 2018)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
