Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathways

Abstract

• COS-5 can increase cell viability and reduce ROS content. • COS-5 can regulate anti-oxidant enzymes system. • COS-5 can inhibit the MMP and the expression of Caspase3. • COS-5 can regulate MAPKs and Nrf2/ARE pathways. Chito-oligosaccharides, the novel marine functional oligosaccharides, possess broad activities including anti-inflammatory and anti-oxidative effects. However, there are few studies about the molecular mechanisms of chito-oligosaccharides monomers against oxidative stress. In this study, the anti-oxidative effect and mechanism of Chitopentaose hydrochloride (COS-5) were explored in HaCaT cells. The results indicated that COS-5 improved cell viability and decreased the production of ROS induced by hydrogen peroxide (H 2 O 2 ). COS-5 can increase the antioxidant-related enzymes including SOD and CAT and can reduce MDA production and LDH leakage in HaCaT cells. COS-5 effectively inhibited the decline of MMP and the expression of Caspase-3 protein. Moreover, COS-5 can significantly down-regulate abnormal activation of MAPKs pathway and up-regulate the Nrf2/ARE pathway. In summary, COS-5 has significant protective effect against H 2 O 2 -induced oxidative stress in HaCaT cells through regulating MAPKs and Nrf2/ARE pathways, suggesting that COS-5 may be used as a novel functional food supplement to prevent oxidative-stress-related diseases in the future.