Abstract
Chitinase enzyme is the N-acetyl D-glucosamine residue group of enzyme responsible for chitin hydrolysis, a linear polymer of β(1,4)nitrogen. Chitinases are wide spread in nature and play a key role in chitin. This study focuses on the isolation of efficient chitinase producing Streptomyces sp. from Western Ghats, a global biodiversity hotspot. A Streptomyces strain TBG AG-28 isolated from the soil sample of Agasthyarkoodam region of Western Ghats, Kerala was found to be an efficient chitinase producer. The strain was identified as Streptomyces sanglieri based on conventional morphological, micro-morphological and 16s rDNA sequence analysis. Different physiological parameters like incubation time, pH and temperature were optimized for maximum production of chitinase under submerged conditions. The highest activity of 42.5 U/mL was attained at seventh day of incubation in colloidal chitin broth with 1% sucrose as carbon source, pH 7 at 32°C. The optimized media showed an increase in activity from 27.5U/mL to 42.5U/mL. Chitinase enzyme was purified using Sephadex G100 column chromatography to 1.84 folds with 6.1% activity recovery. The molecular weight of purified enzyme was found to be 38 kDa by SDS-PAGE analysis and was subsequently characterized.
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