Abstract
In cultured carrot cells, treatment with exogenously supplied ethylene or 1-aminocyclopropane-1-carboxylic acid induced chitinase activity. Most of the induced enzymes were secreted into the culture medium. The extracellular chitinases induced by ethylene were resolved by gel-filtration chromatography into four isoenzymes with different molecular sizes. Two of them were exo-hydrolases which liberated chitobiose as a sole product from insoluble chitin. Another two isomers were thought to be endo-chitinase because they yielded chitin oligomers of various sizes. As major molecular species of chitinase found in ethylene-treated carrot cell cultures were different from those induced by fungal walls, it was suggested that ethylene and fungal components act as independent signals in chitinase induction.
Published Version
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