Abstract
Chitin synthase (UDP-2-acetamido-2-deoxy- d-glucose:chitin 4-β-acetamido-deoxy- d-glucosyltransferase, EC 2.4.1.16) activity from the wall-less variant of Neurospora crassa (slimes) was partially characterized. The slime enzyme activity was found to be similar to that reported for slime-like and wild-type chitin synthase activities with respect to the following: specific activity, particulate cell-fraction localization, activation by N-acetylglucosamine, apparent K m with respect to substrate, pH optimum and ion requirement. It appears that the phenotype of slime cannot be solely accounted for by the absence of chitin synthase enzyme activity.
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