Abstract
This study was conducted to investigate the effects of Chinese medicine granule (CMG, including Cortex Phellodendron, Atractylodes Rhizome, Agastache Rugosa and Gypsum Fibrosum) on absorption and transport of glucose in porcine small intestinal brush border membrane vesicles (BBMVs) under heat stress. Forty-eight 2-month-old Chinese experimental barrows were screened according to weight and litter origin, and then allotted to three groups and treated as follows: Normal temperature control group (NTCG; 23°C), high temperature control group (HTCG; 26°C for 19 h, 40°C for 5 h); Chinese medicine granule anti-stress group (CMGG; 26°C for 19 h, 40°C for 5 h) (n = 16 per group). The results showed that high temperature treatment decreased (p 0.05) in the expression of SGLT1 and GLUT2 genes in the small intestine of pigs compared with the NTCG. Dietary supplementation with CMG improved the growth performance, and increased the activity of disaccharidases in duodenum and jejunum of heat stressed pigs (p 0.05) them in the ileum compared with the HTCG. These results indicated that CMG treatment significantly improved porcine growth performance, and increased intestinal glucose absorption and transport by BBMVs under heat stress, in addition to up-regulating the expression of SGLT1 and GLUT2 genes in porcine duodenum and jejunum.
Highlights
Heat stress causes a series of physiological and metabolic changes in pigs such as elevated body temperature, panting and respiratory alkalosis, and changedTraditional Chinese medicine has been widely used toSong et al (2009) Asian-Aust
A significant elevation in the expression of SGLT1 and GLUT2 genes with the Chinese medicine anti-stress granule group (CMGG) was observed in the duodenum and jejunum compared with the two control groups (p0.05)
This trial investigated the dynamic effect of CMG on porcine growth performance and intestinal enzyme activities on day 1, 3, 6, 10 during high temperature treatment
Summary
Heat stress causes a series of physiological and metabolic changes in pigs such as elevated body temperature, panting and respiratory alkalosis, and changed. Studies on Chinese medicine certified that the active component of Atractylodes Rhizoma, β-Eudesmol, has the function of preventing the evil wetness, and Gypsum Fibrosum is generally used to prevent and cure sthenic fever and domination disease (Chen et al, 2006). The previous study by our laboratory (Liu et al, 2002; Wang et al, 2007) demonstrated that a Chinese medicine prescription, composed of four medicines (including Cortex Phellodendron, Atractylodes Rhizome, Agastache Rugosa and Gypsum Fibrosum), improved the growth performance and immune competence of porcine small intestine in a high temperature environment (40°C). The current study was, designed to investigate changes in activity of functional enzymes and glucose transporter protein expression in intestinal BBMVs of a porcine heat stress model established in our laboratory, and to probe the regulative mechanism of Chinese medicine granule on growth performance and glucose absorption in porcine small intestine under heat stress
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