Year-end Sale % OFF 
Abstract
Nonalcoholic fatty liver disease (NAFLD) is a hepatic ailment with a rapidly increasing incidence in the human population due largely to dietary hyper nutrition and subsequent obesity. Discovering effective natural compounds and herbs against NAFLD can provide alternative and complementary medical treatments to current chemical pharmaceuticals. In this study, ICR male mice were fed a high-fat diet (HFD) in vivo and the AML12 cells were treated with palmitic acid (PA) in vitro. We explore the protective effect and potential mechanism of Chinese Herbal Formula (CHF03) against NAFLD by HE staining, transmission Electron Microscopy assay, Western blotting, and gene expression. In vivo, oxidative stress markers (GSH, GSH-px, MDA, SOD, and CAT) confirmed that CHF03 alleviated oxidative stress and abundance of NF-κB proteins indicating a reduction in inflammation and oxidative stress. The lower protein abundance of ACACA and FASN indicated a preventive effect on lipogenesis. Histological and ultrastructural observations revealed that CHF03 inhibited NAFLD. Expression of Srebf1, Fasn, and Acaca, which are associated with lipogenesis, were downregulated. In vitro, genes and proteins are expressed in a dose-dependent manner, consistent with those in the liver. CHF03 inhibited lipid accumulation and expression of NF-κB, nuclear transfer, and transcriptional activity in AML12 cells. The CHF03 might have a beneficial role in the prevention of hepatic steatosis by altering the expression of lipogenic genes and attenuating oxidative stress.
Highlights
One of the most serious health problems is liver disease, which affects more than 10% of the world’s population (Hong et al, 2015)
The CHF03 mice had 7% lower body weight compared with high-fat diet (HFD) mice
Morphological observation indicated that the appearance of liver in mice fed HFD was hypertrophic and yellow, but the color, size, and weight of liver in mice receiving CHF03 was close to normal (Figure 1B)
Summary
One of the most serious health problems is liver disease, which affects more than 10% of the world’s population (Hong et al, 2015). The most common type of liver disease in industrialized countries is nonalcoholic fatty liver disease (NAFLD). Survey data indicate that NAFLD is the leading cause of chronic liver disease in the United States alone (more than 75%) (Younossi et al, 2011). Unlike other major causes of mortality, liver disease rates are increasing rather than declining (Than and Newsome, 2015). According to the period and severity of liver injury, the types of liver injury can be divided into steatosis, steatohepatitis (KhalatbariSoltani et al, 2019), fibrosis, cirrhosis, and cancer (Byrne and Targher, 2015). NAFLD is caused by nutrient factors and lipid accumulation in biological tissues (Donnelly et al, 2005). Decreased fat production and lipid clearance in the liver can lead to liver steatosis and lipidrelated liver damage (Musso et al, 2009)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
