Abstract
Male screwworm flies, Cochliomyia hominivorax (Coquerel), treated with a sterilizing dose of N,N'-tetramethylenebis(1-aziridinecarboxamide) were sexually sterile about 40 min. after injection, 110 min. after immersion, or 180 min. after topical administration. Flies treated topically retained a considerable external residue 1 week later. At 24 hr posttreatment, about 1/2 the applied dose was present. However, more than 95% of this external residue could be removed by briefly immersing the flies in a strong aqueous solution of sodium thiosulfate. In contrast, flies treated by immersion had no external residue detectable by bioassay 24 hr or 1 week posttreatment. By chemical assay, they had 0–22.2 μg/fly at 8 hr posttreatment, but this amount had dropped to 0–8.6 μg/fly at 24 hr posttreatment.
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