Chemosensitivity and angiogenic potency of pre-B acute lymphoblastic leukemia cell line Sup-B15

Abstract

Objective It has been reported that Ik6 overexpression is associated with poor outcome of childhood pre-B acute lymphoblastic leukemia and Sup-B15 cell line is Ik6 positive. There were fewer studies on the Sup-B15 in the country, so the aim of this research is to explore chemosensitivity and angiogenic potency of pre-B acute lymphoblastic leukemia cell line Sup-B15 associated with Ik6 positive expression through making a comparison between Sup-B15 and Nalm-6 cell line. Methods The Ik6 mRNA and protein expression were detected by nested reverse transcription polymearse chain reaction and Western blot.The effect of vincristine, daunorubicin, L-asparaginase and dexa-methasone on the proliferation of cells was measured by using CCK-8 assay.The migration and invasion of cells were detected by transwell assay and mRNA expression of angiogenesis genes(VEGF, Flt-1, PlGF, IGF-1, Ang1 and Ang2) was measured by real-time PCR. Results No expression of Ik6 was detected in Nalm-6 cell line.Compared with Nalm-6, Sup-B15 cell line had a lower rate of growth.The 4 drugs could inhibit the proliferation of cells in a dose-and time-depen-dence manner.Sup-B15 was more sensitive to daunorubicin, L-asparaginase and dexamethasone, and the folds of respective IC50 value were 9.5, 1.1 and 1.5.But Sup-B15 was more resistant to vincristine than Nalm-6.The result of transwell assay showed that less Sup-B15 cells transmigrated to the lower chamber.A significant lower expression level of angiogenesis genes was detected in Sup-B15 cells. Conclusion These findings suggest that Sup-B15 is more sensitive to chemotherapy and less angiogenic compared with Nalm-6. Key words: Sup-B15; Nalm-6; Chemosensitivity; Invasiveness