Abstract
Two chemometric methods, inverse least square and classical least square, were applied to simultaneous assay of clopidogrel bisulphate and aspirin in their combined dosage tablet formulation. Twelve mixed solutions were prepared for the chemometric calibration as training set and 10 mixed solutions were prepared as validation set. The absorbance data matrix was obtained by measuring the absorbance at 16 wavelength points, from 220 to 250 nm with the interval of 2 nm (Δλ= 2 nm). The developed calibrations were successfully tested for laboratory mixtures as well as commercial tablet formulation for their clopidogrel bisulphate and aspirin concentration. Mean recoveries for clopidogrel bisulphate and aspirin were found to be in good agreement with the label claim.
Highlights
Two chemometric methods, inverse least square and classical least square, were applied to simultaneous assay of clopidogrel bisulphate and aspirin in their combined dosage tablet formulation
Several spectrophotometric[6] and HPLC7-10 methods are reported for the estimation of aspirin in literature, whereas only a few HPLC11-13 methods are available for clopidogrel bisulphate
The multivariate or chemometric methods such as classical least square (CLS), inverse least square (ILS), principal component regression (PCR) and partial least square (PLS), based on computer assisted instrumentation have been applied for the analysis of multicomponent analysis of mixtures[15,16,17]
Summary
Inverse least square and classical least square, were applied to simultaneous assay of clopidogrel bisulphate and aspirin in their combined dosage tablet formulation. The developed calibrations were successfully tested for laboratory mixtures as well as commercial tablet formulation for their clopidogrel bisulphate and aspirin concentration. A spectrophotometric method[14] was reported recently in literature for simultaneously analyzing ASP and CLP where the analysis was done after hydrolyzing the drugs. Two chemometric methods based on ILS and CLS are successfully applied to the simultaneous determination of CLP and ASP in a commercial tablet formulation without any separation procedure. Amount of dissolved drugs in laboratory samples and commercial tablet formulation were calculated by the chemometric methods.
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