Abstract
Ellman's reagent is reduced in two stages by ascorbic acid in the presence of copper ions. The first stage involves some reversible steps and is associated with the formation of dehydroascorbic acid. In this stage, the disulphide compound probably accepts electrons in place of oxygen. The reducing power of 2,3-diketogulonic acid, formed by delactonisation of dehydroascorbic acid, probably effects the second stage of reduction. This is only observed upon addition of EDTA at specified intervals. Two moles of sulphydryl anions are formed per mole of disulphide. The reaction is useful for following the copper catalysed oxidation of low concentrations of ascorbic acid, but is complicated by the effects of copper ion and the hydrogen peroxide formed on the coloured anion.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Chemical Technology and Biotechnology. Chemical Technology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
