Abstract
We have been able to produce chemiluminescence in firefly luciferin without utilizing the enzyme luciferase. Following the analogous mechanism of the chemiluminescence of luminol in the organic solvent dimethyl sulfoxide, we have prepared synthetically the methyl ester of luciferin, the phosphate ester of luciferin and luciferyl adenylate by condensation in dimethyl sulfoxide with Khorana's reagent, dicyclohexylcarbodiimide and diazomethane, phosphoric acid, and adenylic acid respectively. These compounds in dimethyl sulfoxide in the presence of base emit a bright chemiluminescence. Like in vitro enzymatic bioluminescence, the luciferyl adenylate. chemiluminescence emission spectrum is dependent upon pH.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
