Abstract
Instrumentation is developed to apply chemiluminescene as a detector for high-performance liquid chromatography and the lucigenin—base—reductant system is investigated as the post-column chemiluminescent reaction. Dependence of emission intensity on reagent flow-rates, analyte flow-rate, sample size, and delay time is studied. Mixtures of ascorbic acid (5–800 mg/ l) and dehydroascorbic acid (10–100 mg/ l) can be quantified. The system was also used with a mixture of glucose, glucuronic acid, creatinine, and ascorbic acid.
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