Abstract
Reducing agents, especially compounds having an α-hydroxycarbonyl group, give an intense chemiluminescence with luminol in the presence of catalyst in alkaline solution. Based on this reaction, the chemiluminescence detection of corticosteroids and p-nitrophenacyl esters of carboxylic acids has been developed. These compounds were separated by high performance liquid chromatography on a reversed phase column (Prodigy ODS) for corticosteroids with 30% acetonitrile and for p-nitrophenacyl esters with 50% acetonitrile as eluent, and detected by the chemiluminescence reaction with luminol-sodium hydroxide solution containing the catalyst as the post-column reagent. Hexacyanoferrate (III) and hexacyanoferrate (II) were used as catalyst of the reaction. The detection limits of cortisone and p-nitrophenacyl hippurate were 1.1 pmol and 0.8 pmol.
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