Abstract
In this study, a new method has been developed for the simple determination of mepivacaine (carbocaine). The method is based on the enhancement effect of mepivacaine in the chemiluminescence (CL) reaction of tris(1,10 phenanthroline) ruthenium(II) with cerium(IV). A mechanism for the CL reaction has been proposed on the basis of UV-Vis, fluorescent and CL spectra. Under optimum conditions, the CL intensity was proportional to the concentration of the drug in solution over the range 0.45-226.25 μg mL-1 (R2 = 0.9996). The LOD was 0.34 μg mL-1 (S/N = 3). LOD was about 10 times lower than the therapeutic concentration of mepivacaine. The percent of relative standard deviation for determination of 11 replicates at level of 9.05 μg mL-1 and 90.50 μg mL-1 of mepivacaine were 1.8 and 3.7%, respectively. The proposed method was applied successfully for the determination of mepivacaine in human plasma and injectable solutions.
Highlights
Local anaesthetics are drugs that reversibly block the conduction of impulses in the peripheral nervous system
The method is based on the fact that the weak CL signal of Ru(phen)32+ and Ce(IV) is significantly enhanced in the presence of mepivacaine (Scheme 1)
This paper described a CL method for the determination of mepivacaine in plasma samples and injectable solutions
Summary
Local anaesthetics are drugs that reversibly block the conduction of impulses in the peripheral nervous system They vary in their clinical profiles, and these differences may be related to their chemical structures.[1] The amide types are widely used for various local or regional treatments. Intoxications with mepivacaine affect mainly the central nervous system (CNS) and the cardiovascular system.[2] The dose administered and the pharmacokinetic profiles of the drugs determine the concentration and the sensitivity required for the analytical method. Chemiluminescence (CL) methods thanks to their intrinsic advantages in terms of high sensitivity, wide linear dynamic range, and simple instrumentation have become an important and valuable detection method in analytical chemistry. To the best of our knowledge, the CL determination of mepivacaine is not reported in the literature This method has a good LOD, 10 times lower than therapeutic concentration of mepivacaine in human plasma. The proposed method was successfully used for the quantification of mepivacaine in pharmaceuticals and human plasma samples
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