Abstract
The preparation of chemically bonded stationary phases based on a silica support for aqueous exclusion chromatography is discussed. A defined amount of functional groups can be bonded on to the silica surface only if the appropriate silane is prepared prior to binding it to the surface. The properties of the prepared stationary phases, i.e., wettability by water, adsorption of water-soluble polymers, etc., are described. Stationary phases with amide bonds can be used to separate by exclusion chromatography polystyrenes and polyethylene glycols in dimethylformamide as eluent as well as polyethylene glycols and dextrans in water as eluent. The graphs of molecular weight versus elution volume are, with acceptable deviations, independent of the nature of the polymer.
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