Chemical Standardization of Thetran Vithai Kutinir Chooranam-An Antidiabetic Siddha Polyherbal Formulation

Elankani Elankani P,Shakila Shakila R,Pitchiahkumar Pitchiahkumar M,Murugammal Murugammal S,Kabilan N Kabilan

doi:10.5530/pj.2019.11.173

Abstract

Introduction: Belief of general public on herbal drugs led to the stable growth of herbal drug industry thereby necessitated the standardization of herbal drugs and pharmacopoeial standards for their quality control. Aims: Thetran Vithai Kutinir Chooranam (TVKC) is a Siddha polyherbal formulation used for type II diabetes. Aim of the present study is to standardize the drug chemically. Methods and Material: Drug was prepared as per the literature, stored in air tight container and subjected to preliminary phytochemical analysis, physico-chemical, thin layer chromatographic photo documentation, high performance thin layer chromatographic finger printing along with chemical markers such as emodin, chrysophanol and gallic acid, quantitative assay of marker compounds, heavy metal analysis, pesticide residue, aflatoxin and microbial load analysis. Results: The results revealed that the drug contains emodin in minor quantity and gallic acid in considerable quantity. The drug is free from microbial, heavy metal contaminations, aflatoxin and pesticide residues. Conclusion: The derived results could serve as a ready reference for quality control assessment of the drug.

Highlights

Belief of general public on herbal drugs led to the stable growth of herbal drug industry thereby necessitated the standardization of herbal drugs and pharmacopoeial standards for their quality control
The preliminary phytochemical tests showed the presence of steroids, triterpenes, flavonoids, alkaloids, coumarins, quinones, anthraquinones, glycosides, proteins, phenols and tannins
Many phenols reported in T. chebula include ellagic acid, punicalagin, chebulanin, neochebulinic acid, chebulinic acid, 1,2,3,4,6-penta-O-galloyl-H-D-glucose, 1,6-diO-galloyl-D-glucose, casuarinin, 3,4,6-tri-O-galloyl-D-glucose and terchebulin.[13,14,15]

Summary

Introduction

Belief of general public on herbal drugs led to the stable growth of herbal drug industry thereby necessitated the standardization of herbal drugs and pharmacopoeial standards for their quality control. Aims: Thetran Vithai Kutinir Chooranam (TVKC) is a Siddha polyherbal formulation used for type II diabetes. Key Messages: The present study of chemical standardization of Thetran Vithai Kutinir Chooranam, an antidiabetic polyherbal Siddha formulation leads to the quality control standards in terms of preliminary phytochemiicals, physico-chemical parameters, TLC identification, HPTLC finger print profiles and quantification of gallic acid which may be useful for the quality check of commercial drug in future. Four gram of the drug is added with 240 ml of water, boiled, filtered and reduced to 60 ml This 60 ml is prescribed for diabetic adults twice a day.[1] The monographs on T. chebula,[2] Strychnos potatorum[3] are available. The combinations of above four drugs, TVKC is not reported for standardization, authors aimed to standardize

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Results

Conclusion