Abstract
Truffles are the rarest species and appreciated species of edible fungi and are well-known for their distinctive aroma and high nutrient content. However, their chemical composition largely depends on the particularities of their grown environment. Recently, various studies investigate the phytoconstituents content of different species of truffles. However, this research is still very limited for Romanian truffles. This study reports the first complete metabolites profiles identification based on gas chromatography-mass spectrometry (GC-MS) and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QTOF-MS) of two different types of Romania truffles: Tuber magnatum pico and Tuber brumale. In mass spectra (MS) in positive mode, over 100 metabolites were identified from 14 secondary metabolites categories: amino acids, terpenes, alkaloids, flavonoids, organic acids, fatty acids, phenolic acids, sulfur compounds, sterols, hydrocarbons, etc. Additionally, the biological activity of these secondary metabolite classes was discussed.
Highlights
At present, truffles (Tuberaceae family, Tuber genus) are considered an emblem of culinary refinement
In this study, was used a qualitative untargeted metabolomics methodology based on the combination of gas-chromatography coupled with mass spectroscopy (GC-mass spectra (MS)) and electrospray ionization quadrupole timeof-flight mass spectrometry (ESI-QTOF-MS) to analyze the metabolic profiles from two Romanian truffles species with high economic value, namely Tuber magnatum pico and Tuber brumale, or winter truffle
The bioactive compounds screening from the truffles sample were tentatively identified by gas-chromatography coupled with mass spectroscopy (GC-MS) and electrospray ionization-quadrupole time-offlight mass spectrometry (ESI-QTOF-MS) analysis
Summary
Truffles (Tuberaceae family, Tuber genus) are considered an emblem of culinary refinement. The truffle’s growth in natural conditions depends on continuously changing climate conditions causing a restriction of their natural area, which directly influences their prices Preserving truffles and their complex flavor still represents a challenge for the modern food industry, and is most probably the main factor in their market evaluation worldwide [1,3,8,9,10,11,12,13]. In this study, was used a qualitative untargeted metabolomics methodology based on the combination of gas-chromatography coupled with mass spectroscopy (GC-MS) and electrospray ionization quadrupole timeof-flight mass spectrometry (ESI-QTOF-MS) to analyze the metabolic profiles from two Romanian truffles species with high economic value, namely Tuber magnatum pico and Tuber brumale, or winter truffle
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