Abstract
The present study, twenty-five chemical compounds of acetone extract from the Helicteres hirsuta leaves have been investigated for the first time using GC–MS, in which neophytadiene, palmitic acid methyl ester, palmitic acid, phytol, linolenic acid and octadecanoic acid were identified as the major constituents. By using disk–diffusion assay, the results showed that the extract was resistant against 6 studied bacterial strains, including Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus and Staphylococcus aureus. Furthermore, the DPPH and MTT methods were used to identify the antioxidant and cytotoxic activities of the studied extract. As a result, the cytotoxicity of the extract against the Hepatoma (Hep-G2) cell line was indicated with the IC50 of 381.8 ppm whereas this value was 1.223 mg/mL in DPPH inhibition.
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