Chemical distribution of glycosphingolipids in third-instar larval organs of the blowfly, Calliphora vicina (Insecta: Diptera).

Abstract

As a first approach to testing the working hypothesis that glycosphingolipids are functionally involved in the ontogeny of insects, their chemical distribution in larval organs was determined and any stadium-correlated differences documented. Selected organs, i.e., the fatbody, striated muscle, intestinal tract, salivary glands, imaginal discs, and central nervous system, were dissected from seven-day-old larvae of the blowfly, Calliphora vicina, and their glycolipids isolated. Two-dimensional, high-performance thin-layer chromatography was used to separate the neutral and acidic glycolipids of each organ. Significantly different total glycolipid component-patterns were obtained for the individual organs, whereby, except for a number of additional uncharacterized components in the intestinal tract, the neutral glycolipids of all organs were found to be qualitatively similar. However, major quantitative differences between the selected organs were found in their total glycolipid-carbohydrate contents, as well as the respective quantitative neutral glycosphingolipid-component distributions. The acidic glycolipids showed pronounced qualitative as well as quantitative organ-dependent variations. Whereas the highest proportion of uncharged glycolipids was characteristic of the fatbody, a high proportion of zwitterionic glycolipid-components was observed to be typical of the central nervous system and imaginal discs, i.e., of organs persisting during larval life and throughout metamorphosis. Imaginal disc glycolipids were distinguished by their high content of acidic glycolipids, a putative reflection of the functional role of these glycoconjugates in regulated cell reorganization during metamorphosis.