Chemical compounds and Antibacterial activity of (Juniperus phoenicea L. and Rosmarinus officinalis L.) From Algerian Sahara

Abstract

Many plants are used in traditional medicine because they contain several molecules endowed with therapeutic activities. For this purpose, and in the framework of valorisation of the Algerian flora, our objective consists of the study of the chemical composition of two vegetable sources pushing for the spontaneous state in the region of Béchar (R. officinalis L. And J. phoenicea L.), and evaluation of the antibacterial activity by determination of the mini-mum inhibitory concentration (MIC) of the extracts on a set of pathogenic bacteria that are taken from the operating theater (EPH Tourabi Boudjamaa, Béchar, Algeria). The methanol/water (80:20, v/v) for 24h extracts were sub-jected to various phytochemical tests for the colorimetric assay, other frac-tions of methanol/water (80:20, v/v), acetone/water (80:20, v/v), and hot distilled water were used to evaluate the antibacterial activity (Disk diffusion and microdilution methods) against airborne pathogenic microorganisms. Phenolics compound contents in various extracts of R. officinalis and J. phoenecea are 2.12±0.14 and 2.64±0.2 mg GAE/g DW, respectively. The re-sults for flavonoids vary between 4.89±0.39 and 6.09±0,35 mg CE/g DW also respectively. For E. coli, the methanol/water extract of J. phoenecea has a stronger effect with a maximum inhibition of 19 mm in diameter, and 12 mm for for Gram positive (S. aureus). And with a low antibacterial effect of the extract of R. officinalis. The results we obtained in this study reveal the sensi-tivity of the various airborne microorganisms collected from operating thea-ters confirming the effectiveness of the methanol / water extracts of the various plants used especially of J. phoenecea.