Abstract
ABSTRACTPetroleum ether extract from Abutilon theophrasti Medic. leaves was optimized by response surface methodology, and the optimal extraction conditions were as follows: ratio of solvent to material (20.12 mL/g), extraction time (5.45 h), and Soxhlet extraction temperature (61.32°C). And the yield of petroleum ether extract collected in August, September, and October was (2.05 ± 0.02)%, (2.39 ± 0.01)%, and (2.32 ± 0.02)%, respectively. The September and October extracts exhibited a better antioxidant activity, which was proved by DPPH·scavenging ability (IC50 value of 327.5 and 331.5 μg/mL), ABTS·+ scavenging ability (IC50 value of 170.1 and 182.1 μg/mL), and reducing power (0.31 and 0.28 mmol Fe2+/100 μg/mL). Meanwhile, the gas chromatograph-mass spectrometry analysis revealed that the main antioxidant components contained 9, 12, 15-octadecatrienoic acid and 9, 12, 15-octadecatrienoic acid, ethyl ester (Z,Z,Z) in three petroleum ether extracts. Therefore, petroleum ether extract from Abutilon theophrasti Medic. leaves can be a potential resource of natural antioxidants in pharmaceutical, medicine, food, and chemical industries.
Highlights
As an annual herb plant in the Malvaceae family, Abutilon theophrasti Medic. (A. theophrasti) is widely distributed all over tropics and sub-tropics.[1]
In order to evaluate the influence of Soxhlet extraction time on the yield of petroleum ether extract (PEE), extraction time was set at 2, 4, 6, 8, and 10 h with extraction temperature of 50°C and ratio of solvent to material of 16 mL/g
The results indicated that the highest correlation coefficient was found between the content of PEE (CPEE) and ferric-ion reducing antioxidant power (FRAP) activity (1.000, p
Summary
As an annual herb plant in the Malvaceae family, Abutilon theophrasti Medic. (A. theophrasti) is widely distributed all over tropics and sub-tropics.[1]. As an annual herb plant in the Malvaceae family, Abutilon theophrasti Medic. It is well known that low-polarity constituents are a major energy source and containing many biological activities such as antioxidant, anti-inflammatory, and anti-cancer activities.[13,14,15,16] Liu et al.[3] reported that fatty acids from A. theophrasti seeds can be used as antidiuretic, antidote, and antipyretic. Low-polarity compounds from A. theophrasti leaves have not been well studied
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