Abstract

Mosquitoes are the deadliest animals on earth and are the vectors of several neglected tropical diseases. Recently, essential oils have emerged as potential renewable, cost-effective, and environmentally benign alternatives to synthetic pesticides for control of mosquitoes. In this work, thirteen species of Piper were collected from different areas of central Vietnam. The essential oils were obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry. The essential oils were screened for mosquito larvicidal activity against Aedes aegypti. Four of the Piper essential oils showed outstanding larvicidal activity against Ae. aegypti, namely P. caninum, P. longum, P. montium, and P. mutabile, with LC50 and LC90 values less than 10 µg/mL. Multivariate analysis has correlated concentrations of β-caryophyllene, β-bisabolene, α-pinene, and β-pinene with mosquito larvicidal activity.

Highlights

  • The genus Piper is the largest in the Piperaceae and is made up of around 1050 species, many of which are important in traditional medicine and as culinary spices [1]

  • We present the essential oil compositions and mosquito larvicidal activities of several species of Piper growing wild in central Vietnam [32,33]: Piper arboricola C

  • In complete contrast to these previous studies, the essential oil composition of P. sarmentosum essential oil from Da Nang, Vietnam, in this study, was composed of β-bisabolene (40.3%), β-pinene (5.5%), β-caryophyllene (5.5%), and caryophyllene oxide (5.1%) as the major components; neither asaricin, palmitic acid, myristicin, nor benzyl derivatives were observed in the present Vietnamese sample

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Summary

Introduction

The genus Piper is the largest in the Piperaceae and is made up of around 1050 species, many of which are important in traditional medicine and as culinary spices [1]. It is found in China as well as Quảng Ninh, Ninh Bình, Thanh Hóa, Nghệ An, and Kon. Piper nigrum L., Vietnamese names Hồ tiêu and Tiêu, is a widespread liana, believed to have originated in India and Indonesia, but cultivated throughout the tropics, including Vietnam provinces of Quảng Trị, Quảng Nam, Kon Tum, Đắc Lắc, Lâm Đồng, An Giang (Châu Đốc), and Kiên. DC., Vietnamese name Tiêu đỏ, is an understory climber found in China as well as Vietnam, Phú Thọ (Tam Thanh), Ninh Bình, Quảng Nam. Piper sarmentosum Roxb., Vietnamese names Lốt and Trầu già, is an herb growing in forests and wet places near villages, from sea level to 1000 m. Piper peltatum Ruiz & Pav., Pothomorphe peltata (L.) Miq., Pothomorphe umbellata (L.) Miq.) is an understory herb, native to the Neotropics, but introduced to Vietnam

Plant Collection and Essential Oils
Essential Oil Compositions
Larvicidal Activities
Agglomerative
Plant Material
Gas Chromatographic-Mass Spectral Analysis
Mosquito Larvicidal Assay
Statistical Analysis
Conclusions
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