Abstract
Abstract The aim of this study was to investigate the chemical compositions of chalk dust and examine the adverse effects of fine chalk particle matters (PM 2.5 ) on rat alveolar macrophages (AMs) in vitro. Morphologies and element concentrations of chalk particles were analyzed using quantitative energy-dispersive electron probe X-ray microanalysis (ED-EPMA). The oxidative response of AMs exposed to chalk PM 2.5 was measured by luminol-dependent chemiluminescence (CL). The results showed that (1) Chalk dust was mainly composed of gypsum (CaSO 4 ), calcite (CaCO 3 )/dolomite (CaMg(CO 3 ) 2 ), and organic adhesives; (2) Fine chalk particles induced the AM production of CL, which was inhibited by about 90% by diphenyleneiodonium chloride (DPI). Based on these results, we showed that cytotoxicity of chalk PM 2.5 may be related to the reactive oxygen species (ROS) generation.
Published Version
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