Chemical Compositions and Characteristics of Biocalcium from Asian Sea Bass (Lates calcarifer) Scales as Influenced by Pretreatment and Heating Processes.

Abstract

Asian sea bass scales discarded from the fish processing industry contain collagen and calcium. The production of biocalcium can increase their value. The effect of alkaline pretreatment on non-collagenous protein removal from scales was investigated. The alkaline pretreatment of scales was optimal when 2 M NaOH solution was used for 10 min. The impacts of heating processes of varying times on chemical compositions and characteristics of biocalcium (BC) powder from alkali-pretreated scales were also studied. A lower loss of hydroxyproline (HYP) and decreased hardness of scales were obtained when the scales were treated with a boiling process. BC powders from the scales subjected to boiling (B-BC) had higher yield and HYP content than BC powders using a high-pressure heating (HP-BC) process. An augmented heating time (10-30 min) lowered yield, HYP, moisture, and protein contents in BC powder regardless of the heating processes. HP-BC powder had higher ash, calcium, and phosphorus contents than B-BC powder. A whiter color and larger mean particle size were attained for the B-BC powders. X-ray diffractograms revealed that all BC powders had hydroxyapatite, which had a crystallinity of 53.60-66.54%, as a major component. FTIR spectra confirmed that all BC powders comprised proteins and inorganic matter. BC powder from scales with high yield and satisfactory characteristics could be used in calcium supplements.