Abstract
A chemical analysis of Edwardsiella tarda lipopolysaccharide (LPS) preparation used for immunization of eels was performed. The LPS was prepared by phenol-water extraction followed by purification with Cetavlon and high speed centrifugation. Chemical analysis showed 3.6% protein, 0.09% organic phosphorus, 22.0% sugar and 8.3% fatty acids. The monosaccharide composition was : 4.1% 2-keto-3-deoxyhexose (KDO), 1.1% pentose, 12.0% hexose, 0.6% 6-deoxyhexose, 3.9% heptose and 3.9% aminosugar. The fatty acids were analyzed by gas-liquid chromatography (GLC) as their methyl esters and their identities were confirmed by mass spectrometry. The results of the GLC analysis showed that the principal constituents were β-hydroxymyristic acid, myristic acid and palmitic acid. The similar composition of the fatty acids and the aminosugar, glucose, galactose, heptose and KDO shows a taxonomic correlation between Salmonella and E. tarda. The presence of non-hydroxylated fatty acids may decrease the immunogenicity of the LPS preparation.
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