Chemical composition of the lipopolysaccharides from Vibrio anguillarum and Vibrio ordalii.

Abstract

Lipopolysaccharides(LPS)were prepared from different serotypes of Vibrio anguillarum, J-O-1, J-O-2 and J-O-3, and Vibrio ordalii. The LPS fractions were obtained by phenol-water extraction followed by Cetavlon treatment and high speed centrifugation.Chemical analysis of V. anguillarum J-O-1, J-O-2 and J-O-3 showed 2.5%, 4.8%and 1.3% protein;36.3%, 30.0% and 20.0% sugar and 7.1%, 11.3% and 6.6%fatty acids. Chemical analysis of V. ordalii showed 17.5%protein, 13.8% sugar and 1.4% fatty acids. The monosaccharide composition of V. anguillarum J-O-1, J-O-2 and J-O-3 was respectively:0.1%, 0.1% and0.1% 2-keto-3-deoxyhexose(KDO);3.0%, 1.9% and 0.6% pentose;32.6%, 21.6% and 12.6% hexose;10.8%, 7.6% and 2.8% 6-deoxyhexose;4.6%, 7.7% and 7.7% heptose;5.1%, 6.0% and10.2% amino sugar. The monosaccharide composition of V. ordalii LPS was:0.0% KDO, 1.5%pentose, 6.6%hexose, 4.0%6-deoxyhexose, 0.2%heptose and 3.1%amino sugar. The principal fatty acids of the V. anguillarum lipid A were:myristic acid, palmitic acid and stearic acid. The principal fatty acids constituents V. ordalii lipid A were: lauric acid, palmitic acid, stearic acidand arachidic acid.