Abstract
As part of our screening of antioxidant extracts from spices and food plants, the antioxidant activity of the EtHO extract of the flowers of aerial parts of Sedum praealtum was tested. Since the extract showed significant activity, a bioassay-directed fractionation of it was carried out. This investigation resulted in the isolation of kaempferol and quercetin as the principle responsible for the antioxidant activity. The antioxidant ability was quantified using the Fogliano method, which is known as DMPD. Flavonols which were characterized by NMR. This similar antioxidant effect has been obtained on some fruits studied by different authors using this same method.
Highlights
During the history, the use of plants has had important variations
The results of the quantification of the phenolic compounds in the crude extract of S. praealtum analyzed are shown in Table 1, expressed as gallic acid equivalents (GAE) in milligrams per 100 milligrams of extract
The present study was carried out to evaluate the antioxidant effect of ethanolic extract of flowers of Sedum praealtum DC, from the extract, two flavonols were isolated which were characterized by nuclear magnetic resonance (NMR) as kaempferol and quercetin
Summary
The use of plants has had important variations. Antioxidants play a significant role in the prevention of diseases, and have a capacity to reduce oxidative stress by chelating trace elements or scavenging free radicals and protecting antioxidant defenses (Prior et al, 1998; Kaur and Kapoo, 2002). For this reason, it is important to search for alternatives with antioxidant activity, as spices with secondary metabolites with antioxidants activity
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