Abstract
The chemical properties of two strains of tobacco streak virus were compared. The two strains elicited similar symptoms in a limited host range and were also similar in the ratio of nucleoprotein components produced in the Nicotiana species used for virus culture, but they were readily distinguishable serologically and also differed in electrophoretic mobility. Chemically, they had the same RNA content and hypochromicity, and similar nucleotide composition, but the amino acid composition of their coat proteins differed markedly, especially in the contents of alanine, aspartic acid, leucine, proline, and valine. Amino acid content and SDS-polyacrylamide gel electrophoresis analysis indicated molecular weights for the protein subunits of about 28,500–30,000 daltons.
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