Chemical analysis and Biological properties of Terminalia macroptera Guill. & Perr. from Eastern Chad

Abstract

Based on a previous ethnobotanical survey, <em>Terminalia macroptera</em> Guill. & Perr. was selected as its heartwood is traditionally used for infections treatment and as mosquito repellent in the province of Ouaddai (Chad). In our course for a better knowledge of this medicinal plant which could be developed as phytomedicine, it is of great interest to subsequently characterize the chemical profile and bioactivities of this plant. In the present study we develop an Ultrahigh-performance liquid chromatography with diode array detection coupled to electrospray ionization and quadrupole time-of-flight (UHPLC-DAD-ESI/QTOF) technique to characterized on line potentially bioactive compounds. Thirteen compounds were identified in active extracts and fractions from <em>Terminalia macroptera </em>Guill. & Perr. heartwood, namely gallic and ellagic acids derivatives, 23-<em>O</em>-galloyl-terminolic acid, 23-<em>O</em>-galloyl arjunolic acid, 4,4’-dihydroxy-Z-stilbene-<em>O</em>-rutinoside and 3,5,4’-trihydroxy-Z-stilbene-<em>O</em>-rutinoside. Antibacterial properties were evaluated against sensitive and resistant <em>Staphylococcus aureus</em> strains and antioxidant activities using 1,1-diphenyl-2-picryl-hydrazyl (DPPH). Stilbene derivatives, 23-<em>O</em>-galloyl-terminolic acid and 23-<em>O</em>-galloyl arjunolic acid are newly reported in <em>T. macroptera </em>Guill. & Perr.