Abstract
A new method has been developed for the chemical 5′-phosphorylation ofoligonucleotides starting from easily available phosphorous-III compounds using phosphate protecting groups which can be cleaved off via β-elimination. The hydrophobic 2-(p-nitrophenyl)-ethyl group, at the phosphodiester level can serve as a purification handle in reversed phase HPLC. Moreover, the procedure has been adapted to automated DNA synthesis.
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