Abstract
The action of 3 chelating compounds, ethylene diamine tetraacetic acid (EDTA), cyclohexane-1,2-diamine tetraacetic acid (CDTA) and diethylene triamine penta acetic acid (DTPA) on weakening the intercellular cement, the calcium pectate, and inducing cell dissociation in cell suspension culture of Melilotus has been investigated. The action was assessed by determining the percentage of free cells, size and number of cell aggregates in suspension cultures treated with different concentrations of chelating compounds. Application of low Ca level together with proper concentrations of EDTA to the cell cultures promoted cell separation. CDTA gave higher percentage of free cells when applied to the cell cultures in comparison with EDTA and DTPA. It is suggested that beside Ca other components in the intercellular cement are responsible for cell aggregation.
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