Abstract
Thirty-four strains of Vibrio cholerae O1 serotype Ogawa isolated in Lao People's Democratic Republic in 1995 were examined for their biological behaviour and cholera toxin productivity. Biotyping indicated that the 34 strains belonged to the El Tor biotype. All strains were hemolysin positive and resistant to polymyxin B and cholera phage IV. However, 4 strains were negative for the hemolysin activity as determined by the test tube method, although they were positive for the antigenic activity. Prophage typing identified 31 strains of the Celebes type, including 3 cured Celebes type, and 3 Ubol type. All the strains produced detectable levels of cholera toxin; 15 isolates produced more than 100 ng/ml, 10 strains between 10 and 100 ng/ml and 9 strains less than 10 ng/ml, determined by using AKI medium in a stationary test tube. The maximum production was 200 ng/ml.
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