Abstract
This chapter discusses the characterization of transient cerebellar membrane glycoproteins. Transient Con A-binding glycoproteins are massively present in rat cerebellar particulate fractions around the 12th postnatal day. The transient glycoproteins are insoluble in 0.5% Triton X-100 and can be easily prepared by affinity chromatography on Con A-Sepharose in the presence of SDS of the Triton X-100 insoluble material from 12 day old rat cerebella. The collated data suggest that the partner cells of parallel fibers contain Con A-like molecules that are able to recognize the transient glycoproteins by their glycan part. Bl and B2 have been isolated by preparative electrophoresis. Their amino acids and monosaccharide compositions have been determined and antibodies have been raised in rabbits.
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