Abstract
The experiments presented herein were designed to study the molecular basis of the restricted cellular localization and transcriptional regulation of the LH/CG receptor in Leydig cells. Using luciferase fusion constructs transfected into Leydig and Sertoli cell lines, we show that the proximal 186 basepairs (relative to the translation start site) of the 5'-flanking region of the rat LH/CG receptor represent a basal promoter that accounts for the Leydig cell-specific expression of this receptor. A region that confers negative transcriptional regulation by cAMP maps to nucleotides -40 to -70 of this basal promoter. Using mobility shift and deoxyribonuclease footprinting assays, we also report the detection of Leydig cell-specific protein(s) that bind to the basal LH/CG receptor promoter. The binding of this protein(s) to the promoter involves an AP-2 consensus sequence beginning at nucleotide -59 as well as additional sequences that remain to be identified. In spite of the fact that the AP-2 site is involved, the protein-DNA complexes detected in Leydig cells are not recognized by an antibody to AP-2.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call