Abstract
The complete mitochondrial genome of Sirembo imberbis was determined by the bioinformatic assembly of the next generation sequencing (NGS) reads. Total length of the mitogenome was 16,717 bp, which harbors the conserved 13 protein-coding genes (PCGs), 2 ribosomal RNAs (12S and 16S), 22 tRNAs, and two non-coding region (the control region and the origin of light-strand replication). Among 13 protein-coding genes, unusual start codon (GTG) was exclusively identified in COX1, while the incomplete stop codons (TA- or T–) were detected in COX2, COX3, ND2, ND3, ND4 and CytB. As a result of phylogenetic tree, S. imberbis formed a cluster of the family Ophidiidae together with Bassozetus zenkevitchi and Lamprogrammus niger.
Highlights
Sirembo imberbis (Siebold et al 1844) is one of the five recognized species in this genus Sirembo, commonly known as the cusk-ells
Mitochondrial DNA was extracted by a commercial DNA isolation kit (Abcam, UK), which war further sheared by Covaris M220 Focused-Ultrasonicator (Covaris Inc., San Diego, CA, USA)
A phylogenetic tree was built with a minimum evolution (ME) algorithm using MEGA7.0 programs (Kumar et al 2016)
Summary
KEYWORDS Cusk eel; NGS; Ophidiidae; Sirembo imberbis; mitochondrial genome Sirembo imberbis (Siebold et al 1844) is one of the five recognized species in this genus Sirembo, commonly known as the cusk-ells. This species can be distinguished from the other four Sirembo species by additional horizontal rows of dusky blotches on the body (Nielsen et al 2015). They are widely distributed in the Pacific from Korea to the northwestern Australia and the genetic information is required for its scientific management and conservation.
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