Abstract
Antigenic variation of infectious bursal disease virus (IBDV) due to propagation in different host systems (bursa of Fabricius, embryos, or cell cultures) was determined by enzyme-linked immunosorbent assay (indirect and antigen capture) and western blot analysis. To conduct this study, we used 27 non-neutralizing anti-VP 2 monoclonal antibodies, a reference panel of nine neutralizing monoclonal antibodies, and 13 neutralizing anti-IBDV chicken polyclonal antibodies. Changes occurred in neutralizing, cross-reactive, conformation-dependent epitopes on the VP 2 protein of IBDV. Interestingly, non-neutralizing, cross-reactive, conformation-dependent and confirmation-independent epitopes also changed on VP 2 . These epitope changes were directly associated with the method used to propagate IBDV. These results demonstrate that different host systems may play an important role in the antigenicity of IBDV.
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