Abstract
To understand the molecular mechanism of tick blood feeding, an anticoagulant protein, Rhipilin-1, was identified in the tick Rhipicephalus haemaphysaloides. The cDNA sequence of Rhipilin-1 is 620 bp, and it encodes a deduced 164 amino acid protein with a size of 18 kDa. Bioinformatic analysis shows that Rhipilin-1 belongs to the Kunitz-type family of inhibitors, containing one Kunitz domain with high homology to the tissue factor pathway inhibitor (TFPI). The recombinant protein expressed in Escherichia coli delayed normal clotting of rabbit plasma both in the recalcification time (RT) and the activated partial thromboplastin time (APTT) tests. Using RT-PCR, mRNA transcripts of Rhipilin-1 were detected in fed but not in unfed ticks. Disruption of the Rhipilin-1 gene with RNAi led to a 52.7% decrease in the tick attachment rate 24 h after introduction in the rabbit ears and a 21.9% decrease in the average engorged body weight of ticks. These results indicate that Rhipilin-1 is a novel anticoagulant protein involved in tick blood feeding with possible future application as a vaccine candidate. The discovery of Rhipilin-1 is the first report on anticoagulant genes in this species of tick.
Published Version
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