Abstract
Three sorghum cytoplasmic male sterile lines CSV4 A(V), CSV4 A(G1) and CSV4 A(M), grouped as A4, were compared with a milo (A1) and two other non-milo (A2 and A3) cytoplasms for their RFLP patterns of mitochondrial DNA (mtDNA). A 9.7 kb clone from pearl millet mtDNA discriminated each of the three A4 entries whereas other maize and pearl millet mtDNA clones used could not distinguish this group completely. The molecular differences within the A4 cytoplasmic group offer some explanation for the inconsistency in the fertility restoration behaviour of these A4 lines obtained with a definite set of testers in the field.
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