Abstract
A layered construct was developed by combining a porous polymer sheet and a cell sheet as a tissue engineered vascular patch. The primary objective of this study is to investigate the influence of mesenchymal stem cells (MSCs) sheet on the tensile mechanical properties of porous poly-(l-lactide-co-ε-caprolactone) (PLCL) sheet. The porous PLCL sheet was fabricated by the solid-liquid phase separation method and the following freeze-drying method. The MSCs sheet, prepared by the temperature-responsive dish, was then layered on the top of the PLCL sheet and cultured for 2 weeks. During the in vitro study, cellular properties such as cell infiltration, spreading and proliferation were evaluated. Tensile test of the layered construct was performed periodically to characterize the tensile mechanical behavior. The tensile properties were then correlated with the cellular properties to understand the effect of MSCs sheet on the variation of the mechanical behavior during the in vitro study. It was found that MSCs from the cell sheet were able to migrate into the PLCL sheet and actively proliferated into the porous structure then formed a new layer of MSCs on the opposite surface of the PLCL sheet. Mechanical evaluation revealed that the PLCL sheet with MSCs showed enhancement of tensile strength and strain energy density at the first week of culture which is characterized as the effect of MSCs proliferation and its infiltration into the porous structure of the PLCL sheet. New technique was presented to develop tissue engineered patch by combining MSCs sheet and porous PLCL sheet, and it is expected that the layered patch may prolong biomechanical stability when implanted in vivo.
Highlights
Many patients with congenital heart disease are often required to have surgical intervention to close the defect with patch graft [1,2]
We investigated a new approach to develop a tissue engineered patch by combining the scaffold tissue engineering and the cell sheet technology that may offer a mutual benefit between the two methods
SEM images (Figure 1b) showed that the PLCL sheet consists of porous structure with an average pore size of 21 μm 4.5 μm
Summary
Many patients with congenital heart disease are often required to have surgical intervention to close the defect with patch graft [1,2]. The growth potential of currently available patches is low [3,4,5] with several problems. Prosthetic and bovine pericardium patches are potentially calcified due to tissue incompatibility [6,7,8]. Autologous pericardium patches are known for their superior biocompatibility, lower risk of contamination, and low cost [9]. Tissue engineering approaches have a great potential for providing vascular patch with capacity to grow and regenerate [2]. The seeded cells are able to grow and regenerate mature tissue layers resembled to native vascular structure along with the degradation of patch scaffold [22]. Regenerating a complex structure-like vascular through patch tissue engineering have yet successfully achieved in clinical application
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
