Abstract
Abstract Styrene-acrylonitrile copolymer having acrylonitrile (AN) content of 19.4% was fractionated first by size exclusion chromatography (SEC) and SEC fractions were then separated by liquid precipitation chromatography (LPC), followed by SEC (SEC-LPC-SEC). LPC was performed by stepwise gradient elution with an octadecylsilane (ODS) silica column and mobile phase of mixtures of n-hexane and chloroform in which the chloroform content in the mobile phase was increased 2% every 5 min. The copolymer was separated by LPC according to both composition and molecular weight (MW); fractions having higher AN content had higher MW. The hyphenated technique of SEC-LPC-SEC gave detailed information in which the first SEC fractions having higher MW had lower AN content. In the same SEC fraction, the LPC fractions having higher MW had higher AN content.
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