Characterization of stereostructure by X-ray and technology of extracting in combination hydrolysis in situ of acankoreanogenin from leaves of Acanthopanax gracilistylus W. W. Smith

Abstract

To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax gracilistylus W. W. Smith (AGS), the crystal structure was measured with a Bruker APEX-II area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ (ECHS) was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P21, with unit cell parameters: a=(8.3652±0.0006) nm, b=(24.721±0.002) nm, and c=(14.5587±0.0011) nm, α=90 °, β=97.850 (4) °, γ=90 °, V=2982.51 nm3, D c = 1.179 mg/m3, and the molecular number (Z) of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology (CCD-RSM) was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows: for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.