Abstract
This study aims to taxonomic identification, molecular characterization and phylogenetic relationship of spiny lobsters based on mitochondrial cytochrome c oxidase I (COI) and the 16S rRNA genes from Bangladesh waters. A total of 19 barcode sequences (10 partial COI sequences and 9 partial 16S rRNA) has successfully generated from 12 collected spiny lobster samples representing four species belonging family Palinuridae. The average genetic distance was found within and between species were 0.834±0.427 and 17.81±0.830, respectively in COI and 0.107±0.255 and 8.401±2.547, respectively in 16S rRNA genes. The successful amplification rate of 16S rRNA was higher than the COI marker. In the Maximum likelihood (ML) tree, the sequences of the same species were clustered together under a single clade for both COI and 16S rRNA which supports the efficacy of both marker genes in differentiating lobsters’ species.
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